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BioLife Solutions CryoStorCS10 210102細(xì)胞DMSO凍存液

簡(jiǎn)要描述:BioLife Solutions CryoStor CS10 210102細(xì)胞DMSO凍存液:BioLife Solutions CryoStor CS10 細(xì)胞凍存液預(yù)混10% DMSO,適用于高效凍存多種類(lèi)型細(xì)胞。

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  • 更新時(shí)間:2025-02-18
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BioLife Solutions CryoStor CS10 210102細(xì)胞DMSO凍存液介紹:

品牌:BioLife Solutions

貨號(hào):PART No. 210102

容積:VOL. 100mL

型號(hào):CryoStor CS10

BioLife Solutions CryoStor CS10 210102細(xì)胞DMSO凍存液訂購(gòu)信息:

品牌

貨號(hào)

產(chǎn)品描述

規(guī)格

BioLife Solutions

210102

CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液

100mL /瓶

 





CS10 210102細(xì)胞DMSO凍存液
英文名:BioLife Solutions CryoStor CS10 Freeze Media

中文名:BioLife Solutions CryoStor CS10 細(xì)胞DMSO凍存液

BioLife Solutions CryoStor CS10細(xì)胞DMSO凍存液210102 代理銷(xiāo)售歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司  :152 1681 4001。紅榮微再-客服: 316 808 6348  經(jīng)銷(xiāo)商專(zhuān)員

BioLIfe Solutions CryoStor CS10細(xì)胞DMSO凍存液產(chǎn)品系列:

CryoStor®, a series of cell-specific, optimized freeze media, is designed to prepare and preserve cells in ultra low temperature environments (-70ºC to -196ºC); CryoStor®, pre-formulated with DMSO, provides a safe, protective environment for cells and tissues during the freezing, storage, and thawing process. Through modulating the molecular-biological response to the cryopreservation process, CryoStor® provides for enhanced cell viability and functionality while eliminating the need for serum, proteins or high levels of cytotoxic agents.

 

CS10 210102細(xì)胞DMSO凍存液

各種規(guī)格的BioLIfe Solutions CryoStor 細(xì)胞DMSO凍存液。

BioLife Solutions CryoStor®是BioLife公司特別優(yōu)化的細(xì)胞凍存液,在極低溫度 (-70ºC to -196ºC)下準(zhǔn)備和保存細(xì)胞。預(yù)混DMSO,為細(xì)胞和組織的冷凍、儲(chǔ)存和解凍過(guò)程提供安全的保護(hù)環(huán)境。CryoStor®通過(guò)調(diào)控凍存過(guò)程的分子生物學(xué)反應(yīng),無(wú)血清、蛋白或具有高細(xì)胞毒性的試劑,就能增強(qiáng)細(xì)胞活力和功能。

BioLife Solutions CryoStor CS10細(xì)胞凍存液特點(diǎn):

用于細(xì)胞和組織的高級(jí)生物保存液

預(yù)混DMSO

無(wú)血清、無(wú)蛋白

USP級(jí)高質(zhì)量成分、cGMP級(jí)生產(chǎn)、FDA master文件

滅菌、內(nèi)毒素和細(xì)胞功能檢測(cè)

品牌

貨號(hào)

產(chǎn)品描述

規(guī)格

BioLife Solutions

210102

CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液

100mL /瓶

BioLife Solutions

210210

CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液

1000mL/袋

BioLife Solutions

210202

CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液

100mL/袋

BioLife Solutions

210374

CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液

16mL /瓶

BioLife Solutions

210373

CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液

10mL /瓶

BioLife Solutions CryoStor CS10 細(xì)胞DMSO凍存液cGMP級(jí)生產(chǎn)

CS10 210102細(xì)胞DMSO凍存液

BioLife Solutions CryoStor CS10 Freeze Media 使用與凍存方法:

CryoStor® Usage and Cryopreservation Protocol

細(xì)胞準(zhǔn)備

1Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2Centrifuge cells to obtain cell pellet

3 Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.

加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor®

a. Cell concentrations: 0.5-10 x 106

cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor® - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

BioLife Solutions 儲(chǔ)存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

細(xì)胞復(fù)蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.

Passive thaw is not recommended.

9) Dilute cell/CryoStor® mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

BioLife Solutions Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

BioLife Solutions CryoStor CS10細(xì)胞DMSO凍存液210102 代理銷(xiāo)售歡迎您致電

重慶市華雅干細(xì)胞技術(shù)有限公司

重慶市華雅干細(xì)胞技術(shù)有限公司以“專(zhuān)注干細(xì)胞研究,提升干細(xì)胞品質(zhì)”為發(fā)展理念,專(zhuān)業(yè)從事干細(xì)胞基礎(chǔ)研究、干細(xì)胞再生醫(yī)學(xué)臨床應(yīng)用研究及干細(xì)胞技術(shù)服務(wù)

 

 

 

 


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